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The application of microarray technology to the identification of Tc1-like element sequences in fish genomes
Wenne, R.; Handschuh, L.; Pocwierz-Kotus, A.; Urbaniak, R.; Formanowicz, P.; Calkiewicz, J.; Brzozowska, K.; Figlerowicz, M.; Wegrzyn, G.; Wröbel, B. (2011). The application of microarray technology to the identification of Tc1-like element sequences in fish genomes. Mar. Biol. Res. 7(5): 466-477
In: Marine Biology Research. Taylor & Francis: Oslo. ISSN 1745-1000, more

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Keywords
    DNA; Fish; Genomes; Transposons; Marine

Authors  Top 
  • Wenne, R., more
  • Handschuh, L.
  • Pocwierz-Kotus, A.
  • Urbaniak, R.
  • Formanowicz, P.
  • Calkiewicz, J.
  • Brzozowska, K.
  • Figlerowicz, M.
  • Wegrzyn, G.
  • Wröbel, B.

Abstract
    The phylogenetic relationships between Tc1 transposable elements have previously been reported for the genomes of some fish species. However, research in this field has been hindered by the low number of fish genome sequences available in databases. The application of a DNA microarray as a universal tool for Tc1 transposon sequence analysis in fish genomes is described here. A prototype oligonucleotide microarray was constructed and used to compare samples of genomic DNA isolated from selected fish species. These results, combined with earlier reported molecular analysis of Tc1 showed the usefulness of DNA microarray in the screening of transposon sequences.

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