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Candida albicans biofilm formation on peptide functionalized polydimethylsiloxane
De Prijck, K.; De Smet, N.; Rymarczyk-Machal, M.; Van Driessche, G.; Devreese, B.; Coenye, T.; Schacht, E.; Nelis, H.J. (2010). Candida albicans biofilm formation on peptide functionalized polydimethylsiloxane. Biofouling (Print) 26(3): 269-275.
In: Biofouling. Taylor & Francis: Chur; New York. ISSN 0892-7014; e-ISSN 1029-2454, more
Peer reviewed article  

Available in  Authors 

    Candida albicans
Author keywords
    biofilm; Candida albicans; peptide; grafting; Modified Robbins Device

Authors  Top 
  • De Prijck, K., more
  • De Smet, N.
  • Rymarczyk-Machal, M.
  • Van Driessche, G.
  • Devreese, B.
  • Coenye, T., more
  • Schacht, E.
  • Nelis, H.J.

    In order to prevent biofilm formation by Candida albicans, several cationic peptides were covalently bound to polydimethylsiloxane (PDMS). The salivary peptide histatin 5 and two synthetic variants (Dhvar 4 and Dhvar 5) were used to prepare peptide functionalized PDMS using 4-azido-2,3,5,6-tetrafluoro-benzoic acid (AFB) as an interlinkage molecule. In addition, polylysine-, polyarginine-, and polyhistidine-PDMS surfaces were prepared. Dhvar 4 functionalized PDMS yielded the highest reduction of the number of C. albicans biofilm cells in the Modified Robbins Device. Amino acid analysis demonstrated that the amount of peptide immobilized on the modified disks was in the nanomole range. Poly-d-lysine PDMS, in particular the homopeptides with low molecular weight (2500 and 9600) showed the highest activity against C. albicans biofilms, with reductions of 93% and 91%, respectively. The results indicate that the reductions are peptide dependent.

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