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Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia: Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCOgenes
Duperron, S.; Fiala-Médioni, A.; Caprais, J.-C.; Olu, K.; Sibuet, M. (2007). Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia: Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCOgenes. FEMS Microbiol. Ecol. 59(1): 64-70
In: FEMS Microbiology Ecology. Federation of European Microbiological Societies: Amsterdam. ISSN 0168-6496, more
Peer reviewed article

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Keywords
    Bacteria; Sulphide deposits; Sulphur oxides; Symbiosis; Lucinidae Fleming, 1828 [WoRMS]; MED, Eastern Mediterranean [gazetteer]; Marine

Authors  Top | Dataset 
  • Duperron, S.
  • Fiala-Médioni, A.
  • Caprais, J.-C.

Abstract
    Symbioses between lucinid clams (Bivalvia: Lucinidae) and autotrophic sulphide-oxidizing bacteria have mainly been studied in shallow coastal species, and information regarding deep-sea species is scarce. Here we study the symbiosis of a clam, resembling Lucinoma kazani, which was recently collected in sediment cores from new cold-seep sites in the vicinity of the Nile deep-sea fan, eastern Mediterranean, at depths ranging from 507 to 1691 m. A dominant bacterial phylotype, related to the sulphide-oxidizing symbiont of Lucinoma aequizonata, was identified in gill tissue by comparative 16S rRNA gene sequence analysis. A second phylotype, related to spirochete sequences, was identified twice in a library of 94 clones. Comparative analyses of gene sequences encoding the APS reductase α subunit and ribulose-1,5-bisphosphate carboxylase oxygenase support the hypothesis that the dominant symbiont can perform sulphide oxidation and autotrophy. Transmission electron micrographs of gills confirmed the dominance of sulphide-oxidizing bacteria, which display typical vacuoles, and δ13C values measured in gill and foot tissue further support the hypothesis for a chemoautotrophic-sourced host carbon nutrition.

Dataset
  • Bacterial symbionts of macrofauna from the Eastern Mediterranean, the Gulf of Cadiz and the Håkon Mosby Mud Vulcano collected during different cruises between 2003 and 2008 and processed by UPMC and MPIMM, more

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