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Seafood quality analysis: Molecular identification of dominant microbiota after ice storage on several general growth media
Broekaert, K.; Heyndrickx, M.; Herman, L.; Devlieghere, F.; Vlaemynck, G. (2011). Seafood quality analysis: Molecular identification of dominant microbiota after ice storage on several general growth media. Food Microbiol. 28(6): 1162-1169. dx.doi.org/10.1016/j.fm.2011.03.009
In: Food Microbiology. Academic Press. ISSN 0740-0020, more
Peer reviewed article

Also published as
  • Broekaert, K.; Heyndrickx, M.; Herman, L.; Devlieghere, F.; Vlaemynck, G. (2012). Seafood quality analysis: Molecular identification of dominant microbiota after ice storage on several general growth media, in: Broekaert, K. (2012). Molecular identification of the dominant microbiota and their spoilage potential of Crangon crangon and Raja sp.. pp. 29-47, more

Available in Authors 
    VLIZ: Open Repository 231611 [ OMA ]

Keywords
    Biota; Fish storage; Microorganisms; Quality; Seafood products; Marine

Authors  Top 
  • Devlieghere, F., more
  • Vlaemynck, G., more

Abstract
    This study points out the limitations of several general growth media frequently used in seafood research by a systematic identification of the microorganisms on fish samples during ice storage unable to grow on those media. Aerobic psychrotrophic count (APC), replication on various general media and total cultivable microbial community denaturing gradient gel electrophoresis (DGGE) analysis revealed that many potential spoilage microorganisms were overlooked. Those microorganisms overlooked by using only one single growth medium were identified by partial 16S rRNA gene and gyrB gene sequencing. Members of the genera Shewanella, Vibrio, Aliivibrio, Photobacterium, Pseudoalteromonas and Psychrobacter, including Photobacterium phosphoreum, Shewanella baltica and Pseudomonas fluorescens are unable to grow on PCA. APC analysis also confirmed that on plate count agar (PCA) the enumeration of the microbiota was underestimated. Although Long and Hammer agar (LH) and marine agar (MA) obtained the best quantitative (APC analysis) and qualitative (replication and DGGE analyses) results for fish quality analysis, analysts have to keep in mind that some species were also unable to grow on those media, such as Pseudomonas fragi and Acinetobacter sp.

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