IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Sequential signaling crosstalk regulates endomesoderm segregation in sea urchin embryos
Sethi, A.J.; Wikramanayake, R.M.; Angerer, R.C.; Range, R.C.; Angerer, L.M. (2012). Sequential signaling crosstalk regulates endomesoderm segregation in sea urchin embryos. Science (Wash.) 335(6068): 590-593 + supporting online matierials. dx.doi.org/10.1126/science.1212867
In: Science (Washington). American Association for the Advancement of Science: New York, N.Y. ISSN 0036-8075, more
Peer reviewed article  

Available in Authors 

Keyword
    Marine

Authors  Top 
  • Sethi, A.J.
  • Wikramanayake, R.M.
  • Angerer, R.C.
  • Range, R.C.
  • Angerer, L.M.

Abstract
    The segregation of embryonic endomesoderm into separate endoderm and mesoderm fates is not well understood in deuterostomes. Using sea urchin embryos, we showed that Notch signaling initiates segregation of the endomesoderm precursor field by inhibiting expression of a key endoderm transcription factor in presumptive mesoderm. The regulatory circuit activated by this transcription factor subsequently maintains transcription of a canonical Wnt (cWnt) ligand only in endoderm precursors. This cWnt ligand reinforces the endoderm state, amplifying the distinction between emerging endoderm and mesoderm. Before gastrulation, Notch-dependent nuclear export of an essential ß-catenin transcriptional coactivator from mesoderm renders it refractory to cWnt signals, insulating it against an endoderm fate. Thus, we report that endomesoderm segregation is a progressive process, requiring a succession of regulatory interactions between cWnt and Notch signaling.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors