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|Preparation of fish tissues for electron microscopy|
|Olson, K.R. (1985). Preparation of fish tissues for electron microscopy. J. Electron Microsc. Tech. 2(3): 217-228. dx.doi.org/10.1002/jemt.1060020307|
|In: Journal of Electron Microscopy Technique. John Wiley & Sons: London. ISSN 0741-0581, more|
Electron microscopy; Fish; Marine
Many of the techniques applied to study the morphology of lower vertebrates were originally developed for analysis of mammalian tissues. However, not all of these methods, especially those involving tissue perfusion, can be directly applied to piscine tissues because of the considerable physiological differences between fish and mammals. The present paper examines these physiological variables in fish and describes how they might affect morphological studies that employ tissue perfusion. A method for perfusion of fish tissues is described in detail. This procedure can be used for administration of fixatives, chemical markers, and vascular replicating resins.