IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

An in vivo 31P-NMR study of the possible regulation of glycogen phosphorylase a by phosphagen via phosphate in the abdominal muscle of the shrimp Crangon crangon
Kamp, G.; Juretschke, H.P. (1987). An in vivo 31P-NMR study of the possible regulation of glycogen phosphorylase a by phosphagen via phosphate in the abdominal muscle of the shrimp Crangon crangon. Biochim. Biophys. Acta, Mol. Cell Res. 929(2): 121-127. hdl.handle.net/10.1016/0167-4889(87)90166-2
In: Biochimica et Biophysica Acta. Molecular Cell Research. Elsevier: Amsterdam. ISSN 0167-4889, more
Peer reviewed article  

Available in  Authors 

Keyword
    Marine
Author keywords
    NMR, 31P-; Glycogen phosphorylase a; Phosphagen; (Shrimp muscle)

Authors  Top 
  • Kamp, G.
  • Juretschke, H.P.

Abstract
    31P-NMR spectroscopy has been used to determine the concentrations of Pi and phosphagen in the abdominal muscle of the shrimp Crangon crangon at rest and to follow the changes during their recovery after exhaustive work in vivo. Additionally, the effect of physiological Pi concentrations to the phosphorylase a activity has been examined. At rest, the cytoplasmic Pi and phosphagen concentrations were 1 mM and 38 mM, respectively. After exhaustive work the concentrations of both metabolites were in the same range (20 mM). During recovery, the time course of phosphagen regeneration correlates with the decrease of Pi, as the sum of the concentrations of these metabolites is constant. The ATP level was stable throughout the experiment. The phosphorylase a, which was already present in the resting muscle, was virtually inactive at the resting Pi level (1 mM). During work, however, Pi, which is probably generated by phosphagen-ATP splitting, might ensure a sufficient activation of phosphorylase a.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors