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Fixatives, Decalcifiers and Ultrastructure of the organic remnants from mural Nacreous Layers of Fossil Cephalopod Shells
Grégoire, Ch.; Goffinet, G.; Voss-Foucart, M.F. (1983). Fixatives, Decalcifiers and Ultrastructure of the organic remnants from mural Nacreous Layers of Fossil Cephalopod Shells. Bull. Kon. Belg. Inst. Natuurwet. Aardwet. = Bull. - Inst. r. sci. nat. Belg., Sci. Terre 55(4): 8 pp., 3 pls.
In: Bulletin van het Koninklijk Belgisch Instituut voor Natuurwetenschappen. Aardwetenschappen = Bulletin de l'Institut Royal des Sciences Naturelles de Belgique. Sciences de la Terre. KBIN: Brussel. ISSN 0374-6291, more
Peer reviewed article  

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Keywords
    Geological time > Phanerozoic > Geological time > Cenozoic > Tertiary > Cenozoic > Neogene
    Geological time > Phanerozoic > Geological time > Cenozoic > Tertiary > Cenozoic > Neogene > Miocene
    Geological time > Phanerozoic > Geological time > Mesozoic > Cretaceous
    Geological time > Phanerozoic > Geological time > Mesozoic > Jurassic
    Geological time > Phanerozoic > Geological time > Mesozoic > Triassic
    Geological time > Phanerozoic > Paleozoic > Palaeozoic > Carboniferous
    Geological time > Phanerozoic > Paleozoic > Palaeozoic > Carboniferous > Pennsylvanian, Middle
    Geological time > Phanerozoic > Paleozoic > Palaeozoic > Devonian
    Geological time > Phanerozoic > Paleozoic > Palaeozoic > Devonian > Devonian, Middle
    Ammonoidea † [WoRMS]; Cephalopoda [WoRMS]; Nautiloidea [WoRMS]
    Marine

Authors  Top 
  • Grégoire, Ch.
  • Goffinet, G., more
  • Voss-Foucart, M.F.

Abstract
    The ultrastructure of the organic remnants has been compared in the TEM, after decalcification of the mural nacre of ammonites and fossil nautiloids by EDTA, which removes a soluble fraction, and after fixation and decalcification by formaldehyde-cetyl-pyridinium chloride-EDTA (CPC method) and chromium sulphate solutions, which are both considered to insure a better preservation of these organic remains. The loose networks of altered trabeculae, frequently fused into membranes, which constitute the ultrastructure of the fossil organic remnants of nacre after decalcification by EDTA, are also found in the samples treated by the CPC method and by chromium sulphate. Continuous membranes, superimposed on the networks, are especially abundant in the material treated by chromium sulphate. It is concluded that the networks of altered trabeculae are not artifacts, but are the representative ultrastructures of the organic remnants of the nacreous layers in the fossils studied so far. It is suggested that disappearance of EDTA soluble substances does not distinctly alter the ultrastructure of the fossil organic residues.

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