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Bacterial host interaction of GFP-labelled Vibrio anguillarum HI-610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae
Rekecki, A.; Gunasekara, R.A.Y.S.A.; Dierckens, K.; Laureau, S.; Boon, N.; Favoreel, H.; Cornelissen, M.; Sorgeloos, P.; Ducatelle, R.; Bossier, P.; Van Den Broeck, W. (2012). Bacterial host interaction of GFP-labelled Vibrio anguillarum HI-610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae. J. Fish Dis. 35(4): 265-273. hdl.handle.net/10.1111/j.1365-2761.2011.01342.x
In: Journal of Fish Diseases. Blackwell Science: Oxford; London; Edinburgh; Boston; Melbourne. ISSN 0140-7775, more
Peer reviewed article  

Available in Authors 
    VLIZ: Open Repository 257569 [ OMA ]

Keyword
    Marine
Author keywords
    GFP-labelled pathogen; gnotobiotic model system; larviculture; microscopy; ultrastructure

Authors  Top 
  • Rekecki, A., more
  • Gunasekara, R.A.Y.S.A., more
  • Dierckens, K., more
  • Laureau, S.
  • Boon, N., more
  • Favoreel, H., more
  • Cornelissen, M.
  • Sorgeloos, P., more
  • Ducatelle, R., more
  • Bossier, P., more
  • Van Den Broeck, W., more

Abstract
    The location and cell damage caused by Vibrio anguillarum, the causative agent of classical vibriosis, within the developing gut of the newly hatched sea bass, Dicentrarchus labrax (L.), is unknown. A gnotobiotic sea bass model was used to investigate the early interactions of V. anguillarum with sea bass larvae. In the present study, germ-free sea bass larvae were orally exposed to a V. anguillarum HI-610 pathogen labelled with the green fluorescent protein (GFP-HI-610) and sampled at regular intervals. Pathogenic colonization of gut enterocytes was observed 2 h post-exposure (p.e.) and onwards, whereas bacteria within the swim bladder were visualized 48 h p.e and onwards. Ultrastructural findings demonstrated direct bacterial contact with the host cell in the oesophageal mucosa and putative attachment to microvilli of mid- and hindgut enterocytes. The present findings form a starting point for studies assessing the impact of potential candidates (probiotics, prebiotics, antimicrobial peptides) to mitigate bacterial virulence.

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