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Immunohistochemical localization of neuropeptide Y positive cell bodies and fibres in forebrain and pituitary of the African catfish, Clarias gariepinus
Zandbergen, M.A.; Voormolen, A.H.T.; Peute, J.; Kah, O.; Goos, H.J.Th. (1994). Immunohistochemical localization of neuropeptide Y positive cell bodies and fibres in forebrain and pituitary of the African catfish, Clarias gariepinus. Neth. J. Zool. 44(1-2): 43-54
In: Netherlands Journal of Zoology. E.J. Brill: Leiden. ISSN 0028-2960, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Zandbergen, M.A.
  • Voormolen, A.H.T.
  • Peute, J.
  • Kah, O.
  • Goos, H.J.Th.

Abstract
    The present study reveals the topographic distribution of NPY-immunoreactive perikarya and fibres in the forebrain of the African catfish, Clarias gariepinus, at the light microscopic level. Our interest was mainly focussed on the possible participation of NPY in the hypothalamo-hypophysial axis. Different procedures were tested, i.e. on immersion-fixed and paraffin-embedded brains as well as after perfusion- fixation and cryosectioning of the frozen brains. Possible cross-reactivity between the NPY-antiserum and FMRFamide was tested and could be excluded. NPY-immunoreactive peridarya were concentrated in a distinct area of the telencepahalon, i.e. the nucleus entopeduncularis (NE). Especially the frontal and lateral parts of the telecephalon were richly innervated by NPY-ir fibres. A second distinct concentration of NPY-ir fibres was found in the area ventralis pars medialis of the telecephalon, known to contain GnRH-ir perikarya. The nucleus praeopticus posterioris (NPP) was densely innervated by NPY-positive fibres. The pituitary hardly showed innervation by NPY-positive fibres, which were then restricted to the neurointermediate lobe. The proximal pars distalis (PPD), containing the gonadotropic (GTH) cells, was devoid of NPY-ir fibres. The results of this immunohistochemical study indicate the posibility of an indirect regulation of the GTH-cells by NPY at the level of the brain, rather than a direct regulation via innervation by NPY fibres.

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