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Identification of the razor clam species Ensis arcuatus, E. siliqua, E. directus, E. macha, and Solen marginatus using PCR-RFLP analysis of the 5S rDNA region
Fernández-Tajes, J.; Méndez, J. (2007). Identification of the razor clam species Ensis arcuatus, E. siliqua, E. directus, E. macha, and Solen marginatus using PCR-RFLP analysis of the 5S rDNA region. J. Agric. Food Chem. 55(18): 7278-7282. hdl.handle.net/10.1021/jf0709855
In: Journal of Agricultural and Food Chemistry. American Chemical Society: Easton, Pa.,. ISSN 0021-8561, more
Peer reviewed article  

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Keywords
    Identification; Seafood; Species; Traceability; Ensis Schumacher, 1817 [WoRMS]; Marine
Author keywords
    Species identification; Razor clam; Ensis spp; 5S rDNA marker; PCR-RFLP

Authors  Top 
  • Fernández-Tajes, J.
  • Méndez, J.

Abstract
    Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis of the 5S ribosomal DNA region has been applied to the establishment of DNA-based molecular markers for the identification of five razor clam species:? Ensis arcuatus, E. siliqua, E. directus, E. macha, and Solen marginatus. PCR amplifications were carried out using a pair of universal primers from the coding region of 5S rDNA. S. marginatus was simply distinguished by the different size of the amplicons obtained. Species-specific restriction endonuclease patterns were found with the enzymes Hae III for E. arcuatus, E. siliqua, and E. directus, and Acs I for E. macha, and when two enzymes were combined, the four species were also identified. Thus, this work provides a simple, reliable, and rapid protocol for the accurate identification of Ensis and Solen species in fresh and canned products, which is very useful for traceability and to enforce labeling regulations.

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