|Vitamin B12 and marine ecology: IV. The kinetics of uptake, growth and inhibition in Monochrysis lutheri|Droop, M.R. (1968). Vitamin B12 and marine ecology: IV. The kinetics of uptake, growth and inhibition in Monochrysis lutheri. J. Mar. Biol. Ass. U.K. 48(3): 689-733. hdl.handle.net/10.1017/S0025315400019238
In: Journal of the Marine Biological Association of the United Kingdom. Cambridge University Press/Marine Biological Association of the United Kingdom: Cambridge. ISSN 0025-3154, more
57Co-labelled vitamin B12 was used to study the kinetics of vitamin B12 limitation in Monochrysis lutheri in continuous and exponentially growing batch cultures and in washed cell suspensions.Specific growth rate in the chemostats was found not to depend directly on medium substrate concentration but on the concentration within the cells (cell quota), obeying a hyperbolic equation of the form (a — c)d = ab. On the other hand, the over-all specific rate of uptake by exponentially growing cells in very dilute batch cultures obeyed the conventional Langmuir isotherm, (a-cXd + b) = ab, in relation to medium concentration.