IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions
Quéguiner, B.; Leblanc, K.; Cornet-Barthaux, V.; Armand, L.; Fripiat, F.; Cardinal, D. (2011). Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions, in: Ceccaldi, H.J. et al. (Ed.) Global Change: Mankind-Marine Environment Interactions. Proceedings of the 13th French-Japanese Oceanography Symposium. pp. 283-287. hdl.handle.net/10.1007/978-90-481-8630-3_51
In: Ceccaldi, H.J. et al. (Ed.) (2011). Global Change: Mankind-Marine Environment Interactions. Proceedings of the 13th French-Japanese Oceanography Symposium. Springer: Dordrecht. ISBN 978-90-481-8630-3. 447 pp. hdl.handle.net/10.1007/978-90-481-8630-3, more

Available in Authors 
    VLIZ: Open Repository 258376 [ OMA ]

Keyword
    Marine

Authors  Top 
  • Quéguiner, B.
  • Leblanc, K.
  • Cornet-Barthaux, V.
  • Armand, L.
  • Fripiat, F., more
  • Cardinal, D., more

Abstract
    A new method is presented that enables distinguishing between active and non-active cells with regard to biogenic silica deposition during frustule formation in natural communities of siliceous phytoplankton. The PDMPO method is based on the fluorescence of biogenic silica after incubation with the probe. Only those cells that have been depositing silica (by adjunction of intercalary plates during the cell cycle or by depositing a new frustule valve upon cell division) exhibit a typical fluorescence that is proportional to the amount of biogenic silica deposited. This new method has several advantages; it is easy to use at sea, very sensitive, and samples can be conserved for several months without major loss of fluorescence. This method offers new possibilities of investigation of ecophysiological controls within the natural diatom community and will also bring more information to the new generation of sophisticated multi-element multi-species biogeochemical models.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors