IMIS | Flanders Marine Institute

Flanders Marine Institute

Platform for marine research


Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Print this page

Cellular responses to elevated light levels in Fucus spiralis embryos during the first days after fertilization
Coelho, S.; Rijstenbil, J.W.; Sousa-Pinto, I.; Brown, M.T. (2001). Cellular responses to elevated light levels in Fucus spiralis embryos during the first days after fertilization. Plant Cell Environ. 24(8): 801-810
In: Plant, Cell and Environment. Blackwell: Oxford. ISSN 0140-7791; e-ISSN 1365-3040, more
Peer reviewed article  

Available in  Authors 


Authors  Top 
  • Coelho, S.
  • Rijstenbil, J.W., more
  • Sousa-Pinto, I., more
  • Brown, M.T.

    Cellular responses of 1-, 2- and 4-d-old Fucus spiralis embryos subjected to a single dose of elevated photosynthetically active photon flux density (PPFD), with or without ultraviolet (U-V) radiation, were investigated by measuring the effects on the effective quantum yield of photosystem II (DeltaF/F-m') and intracellular production of active oxygen species (AOS). Production of AOS was determined by the in vivo conversion of 5-(and-6)-chloromethyl-2',7-dichlorodihydrofluorescein diacetate (CM-DCFH2-DA) to the fluorescent compound dichlorofluorescein (DCF) using confocal laser scan microscopy (CLSM) and image analysis. The role of xanthophyll cycle pigments in photoprotection was also assessed. A rapid decline in DeltaF /F-m' was observed under all elevated fight conditions. A correlation was found between non-photochemical quenching and the de-epoxidation ratio zeaxanthin/(zeaxanthin + violoxanthin). Active oxygen formation increased with PPFD and was higher in older embryos and when UVB was present. Two photoinhibition responses were recognized: (i) a rapid decline of the PSII yield due to the violoxanthin-zeaxanthin cycle (photoprotection), and (ii) a slower second-phase decline, correlated with active oxygen production. Electron transport rate (ETR) increased with embryo age, and was correlated with AOS production. As a result of enhanced AOS production, there was a slow recovery of the PSH yield, in particular with increased effective UV dose. In general, embryos were able to recover from the imposed light conditions, but UVB had a more damaging effect. Overall, our data suggest that under natural conditions, embryos of F spiralis are susceptible to elevated light levels, and that UVB radiation is an important stress factor.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors