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Allozyme differences between two endangered Mediterranean killifishes, Aphanius iberus and A. fasciatus (Teleostei : Cyprinodontidae)
Maltagliati, F. (1998). Allozyme differences between two endangered Mediterranean killifishes, Aphanius iberus and A. fasciatus (Teleostei : Cyprinodontidae). Ital. J. Zoolog. 65: 303-306
In: Italian Journal of Zoology. Taylor & Francis/Mucchi Editore: Modena. ISSN 1125-0003, more
Peer reviewed article  

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Keywords
    Allozymes; Brackishwater fish; Conservation; Fish; Genetic distance; Genetic diversity; Genetic markers; Mediterranean [Marine Regions]; Brackish water
Author keywords
    Genetic variations; Brackish habitats

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  • Maltagliati, F., more

Abstract
    Cellulose acetate electrophoresis of allozymes was used to differentiate the mio endangered Mediterranean killifishes, Aphanius iberus and A. fasciatus. Thirty-eight putative enzyme-coding loci were examined in two populations from the Albuixec lagoon (Spain) and from the Biguglia coastal pond (Corsica). Low within-population polymorphism was detected. The mean number of alleles per locus, percentage of polymorphic loci, and heterozygosity were A = 1.2 (SE 0.1), P-99 = 21.1%, H = 0.050 (SE 0.018) in A. iberus, and A = 1.3 (SE 0.1), P-99 = 28.9%, H = 0.057 (SE 0.019) in A. fasciatus. A general concordance with the Hardy-Weinberg equilibrium was detected. Allelic distribution for 13 loci proved diagnostic at species level. The genetic distance between the two species analysed was relatively high (D = 0.539), indicating considerable genetic divergence between the mio congeneric species. These results represent a valuable basis for further studies on the genetic structure of Mediterranean cyprinodontids to ensure sustainable management of these species.

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