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Identification of gobies (Teleostei: Perciformes: Gobiidae) from the North and Baltic Seas combining morphological analysis and DNA barcoding
Knebelsberger, T.; Thiel, R. (2014). Identification of gobies (Teleostei: Perciformes: Gobiidae) from the North and Baltic Seas combining morphological analysis and DNA barcoding. Zool. J. Linn. Soc. 172(4): 831–845,. hdl.handle.net/10.1111/zoj.12189
In: Zoological Journal of the Linnean Society. Academic Press: London. ISSN 0024-4082, more
Peer reviewed article  

Available in Authors 

Keywords
    Meristic characters; Morphology; Pomatoschistus Gill, 1863 [WoRMS]; Marine
Author keywords
    COI; Mitochondrial DNA; species identification

Authors  Top 
  • Knebelsberger, T.
  • Thiel, R.

Abstract
    Gobies are difficult to identify, as they are very similar in appearance. Here, we identified (sub)adult specimens of 12 goby species from the North Sea and the Baltic Sea by carefully analysing meristic characters, coloration patterns, papillae row patterns and morphometric measurements. The results of the morphological identifications were congruent with those obtained with the analysis of COI DNA barcodes; sequences from morphological conspecific specimens were clustered together in clades with bootstrap values =?99%. Mean intra- and interspecific distance (uncorrected p) was 0.37 and 18.97%, respectively. A gap between the maximum intraspecific distance and the distance to the nearest neighbour was apparent in every species and ranged from 2.35 to 16.11%. The Barcode Index Number (BIN) analysis performed on the Barcode of Life Data Systems (BOLD) web platform, assigned the DNA barcodes to 12 separate clusters corresponding to sequence- and morphology-based identification. In 25% of the investigated species, the BIN clusters showed taxonomic discordances, as they contained sequences assigned to more than one species. This result demonstrates the importance of accurate morphological species identification at the beginning of the barcoding pipeline.

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