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PCBs do not induce DNA breakage in vitro in human lymphocytes
Belpaeme, K.; Delbeke, K.; Zhu, L.; Kirsch-Volders, M. (1996). PCBs do not induce DNA breakage in vitro in human lymphocytes. Mutagenesis 11(4): 383-389
In: Mutagenesis. United Kingdom Environmental Mutagen Society: Oxford. ISSN 0267-8357, more
Peer reviewed article  

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  • Belpaeme, K., more
  • Delbeke, K., more
  • Zhu, L.
  • Kirsch-Volders, M.

Abstract
    Polychlorinated biphenyls (PCBs) are classified by IARC as non-mutagenic in vivo, However, despite almost 20 years of research, their mutagenicity in vitro is still debatable. In this work the in vitro cytochalasin B micronucleus test and the alkaline comet assay applied to human lymphocytes were used to study the genotoxicity of a PCB, PCB77, at concentrations ranging from 0.01 to 100 µg/ml, was used in whole blood or isolated lymphocyte cultures, with final dimethylsulfoxide percentages of 0.5-2%. In the micronucleus test lymphocytes were exposed for 48 h, and in the alkaline comet assay for 30 min, 1 h and 3 h. No increases of single strand breaks or micronucleus frequencies was found, in contrast to previously reported data, Our data indicate that PCB77 has no clastogenic properties in human lymphocytes.

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