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Analysis of polyphosphates in fish and shrimps tissues by two different ion chromatography methods: Implications on false-negative and -positive findings
Kaufmann, A.; Maden, K.; Leisser, W.; Matera, M.; Gude, T. (2005). Analysis of polyphosphates in fish and shrimps tissues by two different ion chromatography methods: Implications on false-negative and -positive findings. Food Addit. Contam. 22(11): 1073-1082. hdl.handle.net/10.1080/02652030500239565
In: Food Additives and Contaminants. Taylor & Francis: London. ISSN 0265-203X, more
Peer reviewed article  

Available in Authors 

Keywords
    Additives; Fish; Polyphosphates; Marine
Author keywords
    Ion chromatography

Authors  Top 
  • Kaufmann, A.
  • Maden, K.
  • Leisser, W.
  • Matera, M.
  • Gude, T.

Abstract
    Inorganic polyphosphates (di-, tri- and higher polyphosphates) can be used to treat fish, fish fillets and shrimps in order to improve their water-binding capacity. The practical relevance of this treatment is a significant gain of weight caused by the retention/uptake of water and natural juice into the fish tissues. This practice is legal; however, the use of phosphates has to be declared. The routine control testing of fish for the presence of polyphosphates, produced some results that were difficult to explain. One of the two analytical methods used determined low diphosphate concentrations in a number of untreated samples, while the other ion chromatography (IC) method did not detect them. This initiated a number of investigations: results showed that polyphosphates in fish and shrimps tissue undergo a rapid enzymatic degradation, producing the ubiquitous orthophosphate. This led to the conclusion that sensitive analytical methods are required in order to detect previous polyphosphate treatment of a sample. The polyphosphate concentrations detected by one of the analytical methods could not be explained by the degradation of endogenous high-energy nucleotides like ATP into diphosphate, but by a coeluting compound. Further investigations by LC-MS-MS proved that the substance responsible for the observed peak was inosine monophsosphate (IMP) and not as thought the inorganic diphosphate. The method producing the false-positive result was modified and both methods were ultimately able to detect polyphosphates well separated from natural nucleotides. Polyphosphates could no longer be detected (<0.5mg kg-1) after modification of the analytical methodology. The relevance of these findings lies in the fact that similar analytical methods are employed in various control laboratories, which might lead to false interpretation of measurements.

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