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Planctomicrobium piriforme gen. nov., sp. nov., a stalked planctomycete from a littoral wetland of a boreal lake
Kulichevskaya, I.S.; Ivanova, A.; Detkova, E.N.; Rijpstra, W.I.C.; Sinninghe Damsté, J.S.; Dedysh, S.N. (2015). Planctomicrobium piriforme gen. nov., sp. nov., a stalked planctomycete from a littoral wetland of a boreal lake. Int. J. Syst. Evol. Microbiol. 65(5): 1659-1665. dx.doi.org/10.1099/ijs.0.000154
In: International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology: Reading. ISSN 1466-5026, more
Peer reviewed article  

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  • Kulichevskaya, I.S.
  • Ivanova, A.
  • Detkova, E.N.
  • Rijpstra, W.I.C., more
  • Sinninghe Damsté, J.S., more
  • Dedysh, S.N.

Abstract
    An aerobic, budding, non-pigmented and rosette-forming bacterium was isolated from a littoral wetland of a boreal lake located in Valaam Island, northern Russia, and designated strain P3T.Ellipsoidal to pear-shaped cells of this bacterium were covered with crateriform pits and possessed stalks suggesting a planctomycete morphotype. 16S rRNA gene sequence analysis confirmed that strain P3T was a member of the order Planctomycetales and belonged to a phylogenetic lineage defined by the genus Planctomyces, with 89 and 86% sequence similarity to Planctomyces brasiliensis and Planctomyces maris, respectively. Strain P3T was a mildly acidophilic, mesophilic organism capable of growth at pH values between pH 4.2 and 7.1 (with an optimum at pH 6.0–6.5) and at temperatures between 10 and 30 o C (optimum at 20–28 o C).Most sugars, a number of polysaccharides and several organic acids were the preferred growthsubstrates. Compared with Planctomyces brasiliensis and Planctomyces maris, which require NaCl for growth, strain P3T was salt-sensitive and did not develop at NaCl concentrations above 0.5% (w/v). The major fatty acids were C16 : 0 and C16 : 1w7c; the cells also contained significant amounts of C18 : 1w7c and C18 : 0. The major intact polar lipids were diacylglycerol-O-(N,N,Ntrimethyl)homoserine (DGTS) lipids; the major neutral lipids were long-chain 1,(w-1)-diols andC31 : 9 hydrocarbon. The quinone was MK-6, and the G+C content of the DNA was 59.0 mol%.Strain P3T differed from Planctomyces brasiliensis and Planctomyces maris by cell morphology,substrate utilization pattern and a number of physiological characteristics. Based on these data,the novel isolate should be considered as representing a novel genus and species ofplanctomycetes, for which the name Planctomicrobium piriforme gen. nov., sp. nov., is proposed. The type strain is P3T (=DSM 26348T=VKM B-2887T).

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