IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Flow cytometric enumeration of marine viral populations at low abundances
Mojica, K.D.A.; Evans, C.; Brussaard, C.P.D. (2015). Flow cytometric enumeration of marine viral populations at low abundances, in: Mojica, K.D.A. Viral lysis of marine microbes in relation to vertical stratification. pp. 159-171
In: Mojica, K.D.A. (2015). Viral lysis of marine microbes in relation to vertical stratification. PhD Thesis. Universiteit van Amsterdam: Amsterdam. ISBN 978-94-91407-20-8. 247 pp. hdl.handle.net/11245/1.487499, more

Also published as
  • Mojica, K.D.A.; Evans, C.; Brussaard, C.P.D. (2014). Flow cytometric enumeration of marine viral populations at low abundances. Aquat. Microb. Ecol. 71: 203–209. dx.doi.org/10.3354/ame01672, more

Available in Authors 

Author keywords
    Flow cytometry; Low abundance; Virus enumeration

Authors  Top 
  • Mojica, K.D.A., more
  • Evans, C., more
  • Brussaard, C.P.D., more

Abstract
    Flow cytometric enumeration has advanced our ability to analyze aquatic virus samples and thereby our understanding of the ecological role viruses play in the oceans. However, low virus abundances are underestimated using the current flow cytometry (FCM) protocol. Our results revealed that low dilutions (<30-fold) not only decreased the total virus count but also limited the ability to differentiate between virus clusters. Here we report a simple and efficient method optimization for improving virus counts and optical resolution at low abundances. Raising the pH of the Tris-EDTA (TE) buffer to 8.2 successfully countered the effect of insufficient buffering capacity at low dilutions, which is caused by the higher proportion of acidic glutaraldehyde fixative in the final sample. The higher buffer pH did not interfere with virus counts at higher dilutions. We therefore recommend amendment of the standard FCM aquatic virus enumeration protocol using a TE buffer with pH 8.2 as a simple and efficient improvement.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors