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Construction of a high-density genetic map and quantitative trait locus mapping in the sea cucumber Apostichopus japonicus
Tian, M.; Li, Y.; Jing, J.; Mu, C.; Du, H.; Dou, J.; Mao, J.; Li, X.; Jiao, W.; Wang, Y.; Hu, X.; Wang, S.; Wang, R.; Bao, Z. (2015). Construction of a high-density genetic map and quantitative trait locus mapping in the sea cucumber Apostichopus japonicus. NPG Scientific Reports 5(14852 ): 11 pp. http://dx.doi.org/10.1038/srep14852
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322; e-ISSN 2045-2322, more
Peer reviewed article  
Available in  Authors 
Keywords
    Apostichopus japonicus (Selenka, 1867) [WoRMS]
    Marine/Coastal
Authors  Top 
  • Tian, M.
  • Li, Y.
  • Jing, J.
  • Mu, C.
  • Du, H.
  • Dou, J.
  • Mao, J.
  • Li, X.
  • Jiao, W.
  • Wang, Y.
  • Hu, X.
  • Wang, S.
  • Wang, R.
  • Bao, Z.
Abstract
    Genetic linkage maps are critical and indispensable tools in a wide range of genetic and genomic research. With the advancement of genotyping-by-sequencing (GBS) methods, the construction of a high-density and high-resolution linkage maps has become achievable in marine organisms lacking sufficient genomic resources, such as echinoderms. In this study, high-density, high-resolution genetic map was constructed for a sea cucumber species, Apostichopus japonicus, utilizing the 2b-restriction site-associated DNA (2b-RAD) method. A total of 7839 markers were anchored to the linkage map with the map coverage of 99.57%, to our knowledge, this is the highest marker density among echinoderm species. QTL mapping and association analysis consistently captured one growth-related QTL located in a 5cM region of linkage group (LG) 5. An annotated candidate gene, retinoblastoma-binding protein 5 (RbBP5), which has been reported to be an important regulator of cell proliferation, was recognized in the QTL region. This linkage map represents a powerful tool for research involving both fine-scale QTL mapping and marker assisted selection (MAS), and will facilitate chromosome assignment and improve the whole-genome assembly of sea cucumber in the future.
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