IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Production of viable trout offspring derived from frozen whole fish
Lee, S.; Seki, S.; Katayama, N.; Yoshizaki, G. (2015). Production of viable trout offspring derived from frozen whole fish. NPG Scientific Reports 5(16045): 12 pp. hdl.handle.net/10.1038/srep16045
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322, more
Peer reviewed article  

Available in Authors 

Keyword
    Marine

Authors  Top 
  • Lee, S.
  • Seki, S.
  • Katayama, N.
  • Yoshizaki, G.

Abstract
    Long-term preservation of fish fertility is essential for the conservation of endangered fishes. However, cryopreservation techniques for fish oocytes and embryos have not yet been developed. In the present study, functional eggs and sperm were derived from whole rainbow trout that had been frozen in a freezer and stored without the aid of exogenous cryoprotectants. Type A spermatogonia retrieved from frozen-thawed whole trout remained viable after freezing duration up to 1,113 days. Long-term-frozen trout spermatogonia that were intraperitoneally transplanted into triploid salmon hatchlings migrated toward the recipient gonads, where they were incorporated, and proliferated rapidly. Although all triploid recipients that did not undergo transplantation were functionally sterile, 2 of 12 female recipients and 4 of 13 male recipients reached sexual maturity. Eggs and sperm obtained from the salmon recipients were capable of producing donor-derived trout offspring. This methodology is thus a convenient emergency tool for the preservation of endangered fishes.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors