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Stable nuclear transformation system for the coccolithophorid alga Pleurochrysis carterae
Endo, H.; Yoshida, M.; Uji, T.; Saga, N.; Inoue, K.; Nagasawa, H. (2016). Stable nuclear transformation system for the coccolithophorid alga Pleurochrysis carterae. NPG Scientific Reports 6(22252). hdl.handle.net/10.1038/srep22252
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322, more
Peer reviewed article  

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Keywords
    Pleurochrysis carterae (Braarud & Fagerland) T.Christensen, 1978 [WoRMS]; Marine

Authors  Top 
  • Endo, H.
  • Yoshida, M.
  • Uji, T.
  • Saga, N.
  • Inoue, K.
  • Nagasawa, H.

Abstract
    Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum.

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