Stable nuclear transformation system for the coccolithophorid alga Pleurochrysis carterae
Endo, H.; Yoshida, M.; Uji, T.; Saga, N.; Inoue, K.; Nagasawa, H. (2016). Stable nuclear transformation system for the coccolithophorid alga Pleurochrysis carterae. NPG Scientific Reports 6(22252): 10. http://dx.doi.org/10.1038/srep22252
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322; e-ISSN 2045-2322, more
| |
Keywords |
Pleurochrysis carterae (Braarud & Fagerland) T.Christensen, 1978 [WoRMS] Marine/Coastal |
Authors | | Top |
- Endo, H.
- Yoshida, M.
- Uji, T.
|
- Saga, N.
- Inoue, K.
- Nagasawa, H.
|
|
Abstract |
Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum. |
|