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Determination of marine phytoplanktonic biomass
Post, E.; Goeyens, L.; Vandenhoudt, A. (1982). Determination of marine phytoplanktonic biomass, in: Heip, C.H.R. et al. (Ed.) Geconcerteerde Onderzoeksacties Interuniversitaire Actie Oceanologie: eindverslag 3. Biological processes and translocations = Actions de Recherche Concertées Action Interuniversitaire Océanologie: rapport final 3. Biological processes and translocations. pp. 200-213
In: Heip, C.H.R.; Polk, Ph. (Ed.) (1982). Geconcerteerde Onderzoeksacties Interuniversitaire Actie Oceanologie: eindverslag 3. Biological processes and translocations = Actions de Recherche Concertées Action Interuniversitaire Océanologie: rapport final 3. Biological processes and translocations. Programmatie van het Wetenschapsbeleid: Brussel. 226 pp., more

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Authors  Top 
  • Post, E.
  • Goeyens, L., more
  • Vandenhoudt, A.

Abstract
    Comparison between the classical spectrophotometric determinations of chlorophyll a with a fluorometric analysis preceded by high-pressure liquid chromatography, shows that interferences make the classical methods inaccurate. In extreme cases the latter induce an overestimation of the concentration up to 30 % . As the pigments are to be extracted, the extraction efficiencies of three solvents are compared. lt appears that 95 % methanol is superior comparing to DMSO and to 90 % acetone. Moreover, the efficiency improves by using a grinder.

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