IMIS | Flanders Marine Institute

Flanders Marine Institute

Platform for marine research


Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

A plant 35S CaMV promoter induces long-term expression of luciferase in Atlantic salmon
Seternes, T.; Tonheim, T.C.; Myhr, A.I.; Dalmo, R.A. (2016). A plant 35S CaMV promoter induces long-term expression of luciferase in Atlantic salmon. NPG Scientific Reports 6(25096): 6 pp.
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322, more
Peer reviewed article  

Available in  Authors 


Authors  Top 
  • Seternes, T.
  • Tonheim, T.C.
  • Myhr, A.I.
  • Dalmo, R.A.

    The long-term persistence and activity of a naked plasmid DNA (pGL3-35S) containing a luc gene (reporter gene) controlled by a plant 35S CaMV promoter was studied in Atlantic salmon (Salmo salar L.) after injection. Atlantic salmon (mean weight 70 grams) were injected intramuscularly with 100 mu g of plasmid DNA. Blood, different tissues and organs were sampled at different time points up to day 535 after injection. Southern blot analysis suggested the presence of extra-chromosomally open circular, linear and supercoiled topoforms of pGL3-35S at day 150 after injection. At day 536 open circular and supercoiled topoforms were detected. Luciferase activity was detected at the injection site up to 536 days post-injection of pGL3-35S, where it peaked at day 150 and decreased to approximately 17% of its maximum activity by day 536. Our study demonstrated that a plasmid containing the 35S promoter was able to induce expression of a reporter gene/protein in fish in vivo and that the plasmid DNA persisted for a prolonged time after intramuscular injection.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors