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A plant 35S CaMV promoter induces long-term expression of luciferase in Atlantic salmon
Seternes, T.; Tonheim, T.C.; Myhr, A.I.; Dalmo, R.A. (2016). A plant 35S CaMV promoter induces long-term expression of luciferase in Atlantic salmon. NPG Scientific Reports 6(25096): 6 pp. http://hdl.handle.net/10.1038/srep25096
In: Scientific Reports (Nature Publishing Group). Nature Publishing Group: London. ISSN 2045-2322, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Seternes, T.
  • Tonheim, T.C.
  • Myhr, A.I.
  • Dalmo, R.A.

Abstract
    The long-term persistence and activity of a naked plasmid DNA (pGL3-35S) containing a luc gene (reporter gene) controlled by a plant 35S CaMV promoter was studied in Atlantic salmon (Salmo salar L.) after injection. Atlantic salmon (mean weight 70 grams) were injected intramuscularly with 100 mu g of plasmid DNA. Blood, different tissues and organs were sampled at different time points up to day 535 after injection. Southern blot analysis suggested the presence of extra-chromosomally open circular, linear and supercoiled topoforms of pGL3-35S at day 150 after injection. At day 536 open circular and supercoiled topoforms were detected. Luciferase activity was detected at the injection site up to 536 days post-injection of pGL3-35S, where it peaked at day 150 and decreased to approximately 17% of its maximum activity by day 536. Our study demonstrated that a plasmid containing the 35S promoter was able to induce expression of a reporter gene/protein in fish in vivo and that the plasmid DNA persisted for a prolonged time after intramuscular injection.

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