IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Print this page

Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging
Trampe, E.; Kolbowski, J.; Schreiber, U.; Kühl, M. (2011). Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging. Mar. Biol. (Berl.) 158(7): 1667-1675. http://dx.doi.org/10.1007/s00227-011-1663-1
In: Marine Biology: International Journal on Life in Oceans and Coastal Waters. Springer: Heidelberg; Berlin. ISSN 0025-3162; e-ISSN 1432-1793, more
Peer reviewed article  

Available in  Authors 

Keyword
    Marine/Coastal

Authors  Top 
  • Trampe, E.
  • Kolbowski, J.
  • Schreiber, U.
  • Kühl, M., more

Abstract
    We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors