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Recognition of purified beta 1,3/1,6 glucan and molecular signalling in the intestine of Atlantic salmon
Kiron, V.; Kulkarni, A.; Dahle, D.; Vasanth, G.; Lokesh, J.; Elvebo, O. (2016). Recognition of purified beta 1,3/1,6 glucan and molecular signalling in the intestine of Atlantic salmon. Dev. Comp. Immunol. 56: 57-66. dx.doi.org/10.1016/j.dci.2015.11.007
In: Developmental and Comparative Immunology. Elsevier: New York,. ISSN 0145-305X, more
Peer reviewed article  

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Keywords
    Salmo salar Linnaeus, 1758 [WoRMS]; Marine
Author keywords
    Atlantic salmon; Beta-1,3/1,6 glucan; MacroGard (R); C-type lectinreceptor genes; Tagln; Actb; Psma2

Authors  Top 
  • Kiron, V.
  • Kulkarni, A.
  • Dahle, D.
  • Vasanth, G.
  • Lokesh, J.
  • Elvebo, O.

Abstract
    Atlantic salmon was orally intubated with a highly purified ß-glucan product (MacroGard®) to study the recognition of the molecule by the receptor genes, the regulation of the downstream signalling genes and global proteins, and the micromorphological changes in the intestine. The ß-glucan receptor genes of Atlantic salmon, sclra, sclrb, sclrc and cr3, seem to recognize the molecule, and initiate the downstream ITAM-motif signalling, as evident from the significantly high mRNA levels of ksyk, mapkin2, il1b and mip2a levels. Among the altered proteins, the Apoa4 (involved in carbohydrate and lipid metabolism); Tagln, Actb (uptake of ß-glucan); Psma2 (associated with substrate recognition); and Ckt (energy metabolism-related) were the overexpressed ones. The underexpressed proteins included the Uk114, Rpl9, Ctsb and Lgal that are connected to proliferation, LPS-stimulation, Il1b and lactose recognition, respectively. Furthermore, the mRNA levels of igt and the number of immune cells in the distal intestine were found to increase upon ß-glucan uptake by the fish. This study provides some clues on the mechanisms by which the ß-glucan evokes response in Atlantic salmon, particularly at the intestinal level.

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