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Echinochrome A regulates phosphorylation of phospholamban Ser16 and Thr17 suppressing cardiac SERCA2A Ca2+ reuptake
Kim, H.; Youm, J.; Jeong, S.; Lee, S.; Song, I.; Ko, T.; Pronto, J.; Ko, K.; Rhee, B.; Kim, N.; Nilius, B.; Mischchenko, N.; Fedoreyev, S.; Stonik, V.; Han, J. (2015). Echinochrome A regulates phosphorylation of phospholamban Ser16 and Thr17 suppressing cardiac SERCA2A Ca2+ reuptake. Pflügers Arch. Eur. J. Physiol. 467(10): 2151-2163. dx.doi.org/10.1007/s00424-014-1648-2
In: Pflügers Archiv - European Journal of Physiology. Springer: Berlin. ISSN 0031-6768, more
Peer reviewed article  

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Keyword
    Marine
Author keywords
    Echinochrome A; Negative inotropic effect; SERCA2A inhibition;Phospholamban phosphorylation

Authors  Top 
  • Kim, H.
  • Youm, J.
  • Jeong, S.
  • Lee, S.
  • Song, I.
  • Ko, T.
  • Pronto, J.
  • Ko, K.
  • Rhee, B.
  • Kim, N.
  • Nilius, B.
  • Mischchenko, N.
  • Fedoreyev, S.
  • Stonik, V.
  • Han, J.

Abstract
    Echinochrome A (Ech A), a marine bio-product isolated from sea urchin eggs, is known to have cardioprotective effects through its strong antioxidant and ATP-sparing capabilities. However, the effects of Ech A on cardiac excitation–contraction (E-C) are not known. In this study, we investigated the effects of Ech A on cardiac contractility and Ca2+ handling in the rat heart. In ex vivo Langendorff hearts, Ech A (3 µM) decreased left ventricular developing pressure to 77.7?±?6.5 % of basal level. In isolated ventricular myocytes, Ech A reduced the fractional cell shortening from 3.4 % at baseline to 2.1 %. Ech A increased both diastolic and peak systolic intracellular Ca2+ ([Ca2+]i). However, the ratio of peak [Ca]i to resting [Ca]i was significantly decreased. Ech A did not affect the L-type Ca2+ current. Inhibiting the Na+/Ca2+ exchanger with either NiCl2 or SEA400 did not affect the Ech A-dependent changes in Ca2+ handling. Our data demonstrate that treatment with Ech A results in a significant reduction in the phosphorylation of phospholamban at both serine 16 and threonine 17 leading to a significant inhibition of SR Ca2+-ATPase 2A (SERCA2A) and subsequent reduced Ca2+ uptake into the intracellular Ca2+ store. Taken together, our data show that Ech A negatively regulates cardiac contractility by inhibiting SERCA2A activity, which leads to a reduction in internal Ca2+ stores.

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