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Improved detection of beta-N-methylamino-L-alanine using N-hydroxysuccinimide ester of N-butylnicotinic acid for the localization of BMAA in blue mussels (Mytilus edulis)
Andrys, R; Zurita, J; Zguna, N; Verschueren, K.; De Borggraeve, W.M.; Ilag, L (2015). Improved detection of beta-N-methylamino-L-alanine using N-hydroxysuccinimide ester of N-butylnicotinic acid for the localization of BMAA in blue mussels (Mytilus edulis). Anal. Bioanal. Chem. 407(13): 3743-3750. dx.doi.org/10.1007/s00216-015-8597-2
In: Analytical and Bioanalytical Chemistry. Springer-Verlag: Heidelberg. ISSN 1618-2642, more
Peer reviewed article  

Available in Authors 

Keyword
    Marine
Author keywords
    BMAA; Isomers; Neurotoxin; LC-MS/MS; Improved derivatization; Quaternaryammonium

Authors  Top 
  • Andrys, R.
  • Zurita, J.
  • Zguna, N.
  • Verschueren, K.
  • De Borggraeve, W.M.
  • Ilag, L.

Abstract
    beta-N-Methylamino-l-alanine (BMAA) is an important non-protein amino acid linked to neurodegenerative diseases, specifically amyotrophic lateral sclerosis (ALS). Because it can be transferred and bioaccumulated higher up the food chain, it poses significant public health concerns; thus, improved detection methods are of prime importance for the identification and management of these toxins. Here, we report the successful use of N-hydroxysuccinimide ester of N-butylnicotinic acid (C-4-NA-NHS) for the efficient separation of BMAA from its isomers and higher sensitivity in detecting BMAA compared to the current method of choice using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization. Implementation of this efficient method allowed localization of BMAA in the non-visceral tissues of blue mussels, suggesting that more efficient depuration may be required to remove this toxin prior to consumption. This is a crucial method in establishing the absence or presence of the neurotoxic amino acid BMAA in food, environmental or biomedical samples.

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