IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Metabarcoding approach for the ballast water surveillance - An advantageous solution or an awkward challenge?
Zaiko, A.; Martinez, J.; Schmidt-Petersen, J.; Ribicic, D.; Samuiloviene, A.; Garcia-Vazquez, E. (2015). Metabarcoding approach for the ballast water surveillance - An advantageous solution or an awkward challenge? Mar. Pollut. Bull. 92(1-2): 25-34. dx.doi.org/10.1016/j.marpolbul.2015.01.008
In: Marine Pollution Bulletin. Macmillan: London. ISSN 0025-326X, more
Peer reviewed article  

Available in  Authors 

Keyword
    Marine
Author keywords
    Invasive species; Ballast water management convention; COI; RuBisCO;High-throughput sequencing; En-route observation

Authors  Top 
  • Zaiko, A.
  • Martinez, J.
  • Schmidt-Petersen, J., more
  • Ribicic, D.
  • Samuiloviene, A.
  • Garcia-Vazquez, E.

Abstract
    Transfer of organisms with ships' ballast water is recognized as a major pathway of non-indigenous species introduction and addressed in a few recent legislative initiatives. Among other they imply scientific and technical research and monitoring to be conducted in a efficient and reliable way. The recent development of DNA barcoding and metabarcoding technologies opens new opportunities for biodiversity and biosecurity surveillance. In the current study, the performance of metabarcoding approach was assessed in comparison to the conventional (visual) observations, during the en route experimental ballast water survey. Opportunities and limitations of the molecular method were identified from taxonomical datasets rendered by two molecular markers of different degree of universality the universal cytochrome oxydase sub-unit I gene and a fragment of RuBisCO gene. The cost-efficacy and possible improvements of these methods are discussed for the further successful development and implementation of the approach in ballast water control and NIS surveillance.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors