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Restriction fragment length analysis of the cytochrome b gene and muscle fatty acid composition differentiate the cryptic flatfish species Solea solea and Solea aegyptiaca
Boukouvala, E.; Cariani, A.; Maes, G.; Sevilla, R.; Verrez-Bagnis, V.; Jerome, M.; Guarniero, I.; Monios, G.; Tinti, F.; Volckaert, F.A.M.; Bautista, J.; Krey, G. (2012). Restriction fragment length analysis of the cytochrome b gene and muscle fatty acid composition differentiate the cryptic flatfish species Solea solea and Solea aegyptiaca. J. Agric. Food Chem. 60(32): 7941-7948. dx.doi.org/10.1021/jf301250v
In: Journal of Agricultural and Food Chemistry. American Chemical Society: Easton, Pa.,. ISSN 0021-8561, more
Peer reviewed article  

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Keywords
    Solea aegyptiaca Chabanaud, 1927 [WoRMS]; Solea solea (Linnaeus, 1758) [WoRMS]; Marine
Author keywords
    Solea solea; Solea aegyptiaca; species diagnosis; cytochrome b gene;EcoR V restriction fragment length; white muscle fatty acid composition

Authors  Top 
  • Boukouvala, E.
  • Cariani, A.
  • Maes, G., more
  • Sevilla, R.
  • Verrez-Bagnis, V.
  • Jerome, M.
  • Guarniero, I.
  • Monios, G.
  • Tinti, F.
  • Volckaert, F.A.M., more
  • Bautista, J.
  • Krey, G.

Abstract
    Overlapping external morphometric characters easily confound the flatfishes Solea aegyptiaca and Solea solea (Soleidae) in areas of the Mediterranean Sea where both species live in sympatry. This leads to uncertainties in the fisheries and marketing of the species, in addition to misinterpretations in biogeography and conservation studies. This paper describes a simple restriction fragment length-based diagnostic test that differentiates S. solea from S. aegyptiaca, as well as from other species of the Soleidae family. Furthermore, the two species living in sympatry in the Gulf of Kavala (North Aegean Sea, Greece) present significant qualitative differences in muscle fatty acid composition, a property that can also be used to distinguish the two cryptic species.

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