IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Print this page

mtDNA control region and D-HPLC analysis: a method to evaluate the mating system in Syngnathidae (Teleostei)
Sanna, D.; Addis, A.; Biagi, F.; Motzo, C.; Carcupino, M.; Francalacci, P. (2008). mtDNA control region and D-HPLC analysis: a method to evaluate the mating system in Syngnathidae (Teleostei). Mar. Biol. (Berl.) 153(3): 269-275. hdl.handle.net/10.1007/s00227-007-0810-1
In: Marine Biology. Springer: Heidelberg; Berlin. ISSN 0025-3162; e-ISSN 1432-1793, more
Peer reviewed article  

Available in  Authors 

Keyword
    Marine

Authors  Top 
  • Sanna, D.
  • Addis, A.
  • Biagi, F.
  • Motzo, C.
  • Carcupino, M.
  • Francalacci, P.

Abstract
    Mitochondrial DNA (mtDNA) has been used extensively in animals for phylogenetic and population studies but it has been applied only occasionally to mating systems analysis, where nuclear markers are usually preferred. We used denaturing-high performance liquid chromatography (D-HPLC) to detect mitochondrial DNA polymorphisms to assess the genetic mating system in Syngnathus abaster. Our study of ten pregnant males, revealed polygyny in 30% of the males, who carried embryos originating from multiple females. In addition, 30% of the pregnant males carried embryos with the same mitochondrial haplotype. This is not sufficient to demonstrate polyandry but allows a rapid selection and a reduced sample size for further studies. In conclusion, the proposed technique is time- and cost-effective, allows the certain identification of polygyny and provides useful information to study polyandry.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors