|RP-HPLC determination of phytoplankton pigments—comparison of calibration results for two columns|Ston-Egiert, J.; Kosakowska, A. (2005). RP-HPLC determination of phytoplankton pigments—comparison of calibration results for two columns. Mar. Biol. (Berl.) 147(1): 251-260. hdl.handle.net/10.1007/s00227-004-1551-z
In: Marine Biology. Springer: Heidelberg; Berlin. ISSN 0025-3162, more
|Authors|| || Top |
- Ston-Egiert, J.
- Kosakowska, A.
In the experiment reported the results obtained with two different columns for the RP-HPLC analysis of phytoplankton pigment components were compared. LiChroCART Hypersil ODS and LiChroCART LiChrospher 100 RP18e columns were used exchangeably. Two groups of high-purity pigment standards of reference phytoplankton strains (23 chlorophylls and carotenoids in the first case and 25 in the second case) were subjected to chromatographic analysis. All calibration parameters (retention times, calibration curves, spectrum shapes, detection thresholds) for qualitative and quantitative identification of pigments were determined. The results indicate that both columns used in the analysis ensure sufficient resolution and can be used interchangeably for the analysis of pigments present in naturally existing phytoplankton, even when present in only trace amounts.