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Double-labelling methods used to diagnose apoptotic and necrotic cell degradations in copepod nauplii
Poulet, S.A.; Richer de Forges, M.; Cueff, A.; Lennon, J.-F. (2003). Double-labelling methods used to diagnose apoptotic and necrotic cell degradations in copepod nauplii. Mar. Biol. (Berl.) 143(5): 889-895. hdl.handle.net/10.1007/s00227-003-1142-4
In: Marine Biology. Springer: Heidelberg; Berlin. ISSN 0025-3162, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Poulet, S.A.
  • Richer de Forges, M.
  • Cueff, A.
  • Lennon, J.-F.

Abstract
    Two double-labelling methods, Tunel+propidium iodide and Annexin V-FITC+propidium iodide, have been tested to diagnose cell degradation processes in N1–N2 nauplius stages produced by spawning females of the copepod Calanus helgolandicus fed either non-toxic (PRO: control) or toxic (TR1) diets under laboratory conditions. Observation of labelled samples with a confocal laser-scanning microscope revealed that the maternal-food effect, following absorption of the toxic diet, induced cell apoptosis and necrosis in 80–100% of the offspring, which sooner or later died. Similar cell damages were also observed in 18–50% of the nauplii produced by females incubated in filtered seawater. Such cell degradation processes and high mortality were not observed in nauplii produced by females fed the non-toxic diet. Our protocols allowed detection of cell degradations before death of unhealthy larvae, preferentially with a confocal microscope or a conventional visible-fluorescent-light microscope.

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