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Participation of the C-type hemolymph lectin in mineralization of the acorn barnacle Megabalanus rosa
Kamiya, H.; Jimbo, M.; Yako, H.; Muramoto, K.; Nakamura, O.; Kado, R.; Watanabe, T. (2002). Participation of the C-type hemolymph lectin in mineralization of the acorn barnacle Megabalanus rosa. Mar. Biol. (Berl.) 140(6): 1235-1240. hdl.handle.net/10.1007/s00227-002-0789-6
In: Marine Biology. Springer: Heidelberg; Berlin. ISSN 0025-3162, more
Peer reviewed article  

Available in Authors 

Keyword
    Marine

Authors  Top 
  • Kamiya, H.
  • Jimbo, M.
  • Yako, H.
  • Muramoto, K.
  • Nakamura, O.
  • Kado, R.
  • Watanabe, T.

Abstract
    BRA-2, a major C-type lectin in the hemolymph of the acorn barnacle Megabalanus rosa was detected in shell-associated proteins. Immobilized BRA-2 promoted CaCO3 crystallization as determined by an in vitro pH-drift mineralization assay. In nature, calcification occurs as wound repair in M. rosa after breakage of the shell plate. To observe this phenomenon, we inserted a rubber cube inside the mantle cavity of M. rosa through the basal plate, and kept the organisms in a tank for several weeks. The rubber cube was covered first with brownish-colored material, probably a melanin, and then with CaCO3 5 weeks after the insertion. In the CaCO3-associated proteins, BRA-3, a minor component of M. rosa hemolymph lectins, was present in addition to BRA-2. The presence of both the lectins and their ligands on the hemocyte was also observed. These results suggest that the C-type lectins of the M. rosa hemolymph participate in mineralization as well as defense in these organisms.

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