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Bioluminescence of Vibrio fischeri in continuous culture: Optimal conditions for stability and intensity of photoemission
Scheerer, S.; Gomez, F.; Lloyd, D. (2006). Bioluminescence of Vibrio fischeri in continuous culture: Optimal conditions for stability and intensity of photoemission. J. microbiol. methods 67(2): 321-329. hdl.handle.net/10.1016/j.mimet.2006.04.010
In: Journal of Microbiological Methods. Elsevier: Amsterdam. ISSN 0167-7012, more
Peer reviewed article  

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Keywords
    Bioluminescence; Continuous culture; Vibrio fischeri (Beijerinck, 1889) Lehmann & Neumann, 1896 [WoRMS]; Marine
Author keywords
    Vibrio fischeri

Authors  Top 
  • Scheerer, S.
  • Gomez, F.
  • Lloyd, D.

Abstract
    Continuous cultivation of the bioluminescent bacterium, Vibrio fischeri NRRL-B-11177, in a fermenter provided a constant supply of bacteria in exponential growth phase. These bacteria may be used as stable bioindicators to measure perturbation of the metabolic state by physical and chemical agents. However, optimization of light emission necessitates careful choice of growth medium and culture operating conditions. Optimized conditions that supported long-term cultivation of V. fischeri NRRL-B-11177 with stable intense bioluminescence were: a dilution rate of 0.08-0.09 h-1, air supply of 600 ml-1, stirring of 300-350 rpm at a constant incubation temperature within the range of 20 to 26 °C and pH 7.8. These conditions were as successful in a 500-ml as in a 10-ml fermenter. This system provided a reliable long-term (more than 1 month) continuous culture facility for the reproducible measurement of perturbation of V. fischeri by monitoring changes in luminescence.

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