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Determination of okadaic acid by micellar electrokinetic chromatography with ultraviolet detection
Bouaïcha, N.; Hennion, M.-C.; Sandra, P. (1997). Determination of okadaic acid by micellar electrokinetic chromatography with ultraviolet detection. Toxicon 35(2): 273-281. dx.doi.org/10.1016/S0041-0101(96)00069-4
In: Toxicon. Elsevier: Oxford. ISSN 0041-0101, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Bouaïcha, N.
  • Hennion, M.-C.
  • Sandra, P.

Abstract
    Micellar electrokinetic chromatography (MEKC) with ultraviolet (UV) detection was applied for the determination of non-derivatized phycotoxins associated with diarrhoetic shellfish poisoning. A detection limit for 40 pg of okadaic acid (OA) was achieved. The UV intensities of this toxin measured at 200 nm showed good linearity in the range 40–640 pg. OA was detected in mussels spiked with 10 ng/g whole tissue. The presence of OA and dinophysistoxin-2 was observed in the crude extract of the dinoflagellate Prorocentrum lima.

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