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Perfusion of gills isolated from the hyper-hyporegulating crab Pachygrapsus marmoratus (Crustacea, Decapoda): adaptation of a method
Pierrot, C.; Péqueux, A.; Thuet, P. (1995). Perfusion of gills isolated from the hyper-hyporegulating crab Pachygrapsus marmoratus (Crustacea, Decapoda): adaptation of a method. Arch. Physiol. Biochem. 103(4): 401-409. dx.doi.org/10.3109/13813459509047129
In: Archives of Physiology and Biochemistry. Swets & Zeitlinger/Taylor & Francis: Lisse. ISSN 1381-3455, more
Peer reviewed article  

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Keywords
    Pachygrapsus marmoratus (Fabricius, 1787) [WoRMS]; Marine
Author keywords
    PACHYGRAPSUS MARMORATUS; OSMOREGULATION; GILL; PERFUSION

Authors  Top 
  • Pierrot, C.
  • Péqueux, A.
  • Thuet, P.

Abstract
    This paper reports the adaptation of a branchialperfusion technique to the gills of the small hyper-hyporegulating crab, Pachygrapsus marmoratus. The physiological quality of the preparation has been established and preliminary measurements of the transepithelial potential difference (PD) and sodium fluxes were reported. A clear-cut distinction has to be made between anterior and posterior gills. With symmetrical bathing conditions (same saline on both sides of the epithelium j, a significant transepithelial PD was measured only in posterior gills isolated from crabs acclimated to dilute sea water. This hemolymph-side negative PD is shown to require biological energy and to be sensitive to ouabain. Na+ influx was larger than efflux, indicating the occurrence of a net inward of Na+. The active nature of the Na+ influx was established.

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