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Nature and distribution of Gonadotropin-Releasing Hormone (GnRH) in the brain, and GnRH and GnRH binding activity in serum of the spotted dogfish Scyliorhinus canicula
D'Antonio, M.; Vallarino, M.; Lovejoy, D.A.; Vandesande, F.; King, J.A.; Pierantoni, R.; Peter, R.E. (1995). Nature and distribution of Gonadotropin-Releasing Hormone (GnRH) in the brain, and GnRH and GnRH binding activity in serum of the spotted dogfish Scyliorhinus canicula. Gen. Comp. Endocrinol. 98(1): 35-49. dx.doi.org/10.1006/gcen.1995.1042
In: General and Comparative Endocrinology. Elsevier: New York,. ISSN 0016-6480, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • D'Antonio, M.
  • Vallarino, M.
  • Lovejoy, D.A.
  • Vandesande, F.
  • King, J.A.
  • Pierantoni, R.
  • Peter, R.E.

Abstract
    The distribution of different molecular forms of gonadotropin-releasing hormone (GnRH) in the brain and serum of the spotted dogfish, Scyliorhinus canicula, was investigated by an indirect immunofluorescence method, using antisera against salmon (s-), chicken-II (cII-) and mammalian (m-) GnRHs, and by reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with radioimmunoassays. Five GnRH molecular forms were demonstrated on the basis of the retention time in the RP-HPLC system. The characterestics of four of these GnRH peptides are consistent with those of m-, cII-, dogfish (df-), and sGnRH. The fifth form appears to be novel. Immunoreactive sGnRH structures were confined to the diencephalon; whereas cIIGnRH and mGnRH were found in the telecephalon and diencephalon, cIIGnRH-and dfGnRH-like molecules were detected in the serum. Moreover, specific, low-affinity GnRH binding protein (GnRH-BP) was found in the serum of the spotted dogfish. The binding of [125I]sGnRHA to the serum GnRH-BP was dependent on incubation time, equilibrium being reached within 1 hr at 4° binding was rapid and incompletely reversible. Scatchard analysis yielded a linear plot with a Kd of 7.9 × 10-7M. The presence of a Gnrh-BP in spotted dogfish serum suggests a probable action of GnRH via the general circulation.

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