IMIS | Flanders Marine Institute

Flanders Marine Institute

Platform for marine research


Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Print this page

Preparation of bromine-77 labeled proteins using bromoperoxidase
Lambert, F.; Slegers, G. (1994). Preparation of bromine-77 labeled proteins using bromoperoxidase. Appl. Radiat. Isot. 45(1): 11-15.
In: Applied Radiation and Isotopes. Elsevier: Amsterdam. ISSN 0969-8043; e-ISSN 1872-9800, more
Peer reviewed article  

Available in  Authors 


Authors  Top 
  • Lambert, F.
  • Slegers, G.

    The halogenating enzyme bromoperoxidase (BPO, EC, isolated from the brown algae Ascophyllum nodosum, was used to catalyze the radiobromination of an anti-hPLAP monoclonal antibody. Radiobromination of bovine serum albumin was also performed and used as control method. The proteins are labeled with bromine-77, produced by the 75As (α, 2n) 77Br reaction.The essential reaction parameters, such as concentration of protein and hydrogen peroxide, the amount of bromoperoxidase, the pH of the reaction mixture, the reaction time and temperature were optimized.The immunoreactivity of the radiolabeled monoclonal antibody was checked with a “Sandwich” ELISA.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors