IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Preparation of bromine-77 labeled proteins using bromoperoxidase
Lambert, F.; Slegers, G. (1994). Preparation of bromine-77 labeled proteins using bromoperoxidase. Appl. Radiat. Isot. 45(1): 11-15. dx.doi.org/10.1016/0969-8043(94)90141-4
In: Applied Radiation and Isotopes. Elsevier: Amsterdam. ISSN 0969-8043, more
Peer reviewed article  

Available in Authors 

Keyword
    Marine

Authors  Top 
  • Lambert, F.
  • Slegers, G.

Abstract
    The halogenating enzyme bromoperoxidase (BPO, EC 1.11.1.7), isolated from the brown algae Ascophyllum nodosum, was used to catalyze the radiobromination of an anti-hPLAP monoclonal antibody. Radiobromination of bovine serum albumin was also performed and used as control method. The proteins are labeled with bromine-77, produced by the 75As (α, 2n) 77Br reaction.The essential reaction parameters, such as concentration of protein and hydrogen peroxide, the amount of bromoperoxidase, the pH of the reaction mixture, the reaction time and temperature were optimized.The immunoreactivity of the radiolabeled monoclonal antibody was checked with a “Sandwich” ELISA.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors