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Detection of mRNA encoding an antibacterial-metalloprotein (MPII) by in situ hybridization with a cDNA probe generated by polymerase chain reaction in the worm Nereis diversicolor
Salzet-Raveillon, B.; Rentier-Delrue, F.; Dhainaut, A. (1993). Detection of mRNA encoding an antibacterial-metalloprotein (MPII) by in situ hybridization with a cDNA probe generated by polymerase chain reaction in the worm Nereis diversicolor. Cell. Mol. Biol. 39(1): 105-114
In: Cellular and Molecular Biology. C.M.B. Association: Oxford. ISSN 0145-5680, more
Peer reviewed article  

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Keyword
    Marine
Author keywords
    PCR; IN-SITU HYBRIDIZATION; ANTIBACTERIAL-METALLOPROTEIN;HEMERYTHRIN-MYOHEMERYTHRIN FAMILY

Authors  Top 
  • Salzet-Raveillon, B.
  • Rentier-Delrue, F.
  • Dhainaut, A.

Abstract
    Based on partial amino acid sequence of an antibacterial-metalloprotein (MPII) consisting of a N-terminal fragment(1-33 th) and an 43 amino acids long internal fragment, two oligonucleotides primers were synthesized and used to generate a cDNA fragment by the PCR method. The specificity of the PCR synthesized 220 bp fragment was verified by hybrid-arrest translation and sequencing. In situ hybridization performed with this cDNA fragment on Nereis diversicolor whole body defined two specific sites of recognition: a cluster of cells floating in the coelom and two types of muscles (perineural and oblique). Finally, the chronological expression of the MPII was postulated.

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