|Detection of mRNA encoding an antibacterial-metalloprotein (MPII) by in situ hybridization with a cDNA probe generated by polymerase chain reaction in the worm Nereis diversicolor|
Salzet-Raveillon, B.; Rentier-Delrue, F.; Dhainaut, A. (1993). Detection of mRNA encoding an antibacterial-metalloprotein (MPII) by in situ hybridization with a cDNA probe generated by polymerase chain reaction in the worm Nereis diversicolor. Cell. Mol. Biol. 39(1): 105-114
In: Cellular and Molecular Biology. C.M.B. Association: Oxford. ISSN 0145-5680, more
PCR; IN-SITU HYBRIDIZATION; ANTIBACTERIAL-METALLOPROTEIN;HEMERYTHRIN-MYOHEMERYTHRIN FAMILY
|Authors|| || Top |
- Salzet-Raveillon, B.
- Rentier-Delrue, F.
- Dhainaut, A.
Based on partial amino acid sequence of an antibacterial-metalloprotein (MPII) consisting of a N-terminal fragment(1-33 th) and an 43 amino acids long internal fragment, two oligonucleotides primers were synthesized and used to generate a cDNA fragment by the PCR method. The specificity of the PCR synthesized 220 bp fragment was verified by hybrid-arrest translation and sequencing. In situ hybridization performed with this cDNA fragment on Nereis diversicolor whole body defined two specific sites of recognition: a cluster of cells floating in the coelom and two types of muscles (perineural and oblique). Finally, the chronological expression of the MPII was postulated.