|Protein kinase C and ion transport in the posterior gills of the Chinese crab Eriocheir sinensis|Asselbourg, V.; Trausch, G.; Gilles, R.; Devos, P. (1991). Protein kinase C and ion transport in the posterior gills of the Chinese crab Eriocheir sinensis. Mar. Biol. (Berl.) 109(1): 115-119. dx.doi.org/10.1007/BF01320237
In: Marine Biology. Springer: Heidelberg; Berlin. ISSN 0025-3162, more
|Authors|| || Top |
- Asselbourg, V.
- Trausch, G.
- Gilles, R., more
- Devos, P.
The possible involvement of protein kinase C in control of ion transport was investigated on a preparation of isolated, perfused posterior gills of the Chinese crabsEriocheir sinensis (collected in 1989 from lakes near Emden, northern Germany) acclimated to fresh water. 1-oleyl-2-acetyl-sn-glycerol (OAG) and phorbol 12-myristate 13-acetate (PMA), two activators of protein kinase C, when added to the perfusion saline, induced depolarisation of the transepithelial potential difference (PD) and an increase in transepithelial Na+ influx. The observed increase was proportional to OAG concentration up to 250µM, with a 2.5× accelerated Na+ influx. OAG and PMA remained without effect on Cl− fluxes. The observed effects were in agreement with an activation, via protein kinase C, of the Na+/K+ ATPase located on the serosal side of the epithelium.