IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (1): add | show Print this page

one publication added to basket [262081]
The use of sea urchin eggs as a model to investigate the effects of crassolide, a diterpene isolated from a soft coral
Pesando, D.; Graillet, C.; Braekman, J.-C.; Dubreuil, A.; Girard, J.-P.; Puiseux-Dao, S. (1991). The use of sea urchin eggs as a model to investigate the effects of crassolide, a diterpene isolated from a soft coral. Toxicology in vitro 5(5-6): 395-401. https://dx.doi.org/10.1016/0887-2333(91)90060-Q
In: Toxicology in vitro. ISSN 0887-2333, more
Peer reviewed article  

Available in  Authors 

Keyword
    Marine/Coastal

Authors  Top 
  • Pesando, D.
  • Graillet, C.
  • Braekman, J.-C.
  • Dubreuil, A.
  • Girard, J.-P.
  • Puiseux-Dao, S.

Abstract
    Crassolide, a monocyclic diterpene isolated and purified from the soft coral Lobophytum crassum, inhibited the cell cleavage of sea urchin eggs without affecting fertilization. The effect was observed with concentrations above 2 × 10−5m in egg suspensions. Addition of crassolide between 5 and 40 min post-fertilization totally blocked the first cleavage, which in the control occurs 1 hr after fertilization. When added between 50 and 60 min post-fertilization, crassolide produced polynucleated cells in embryos. Crassolide did not affect the egg permeability to Na+ and Ca2+, but caused an increase of 0.2 units in the intracellular pH of fertilized eggs coupled with a proton efflux. Crassolide, which does not affect Ca2+ influx or permeability at the level of storage in reticular vesicles, could be used as a negative control when analysing calcium changes in short-term toxicological studies. The relationship between the pH increase and the cell cleavage needs further investigation.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors