Glycerol-3-phosphatase and the control of glycerol metabolism in Dunaliella tertiolecta cells
Belmans, D.; Van Laere, A. (1988). Glycerol-3-phosphatase and the control of glycerol metabolism in Dunaliella tertiolecta cells. Arch. Microbiol. 150(2): 109-112. https://dx.doi.org/10.1007/BF00425148
In: Archives of Microbiology. Springer: Heidelberg; Berlin. ISSN 0302-8933; e-ISSN 1432-072X, more
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| Keywords |
Dunaliella tertiolecta Butcher, 1959 [WoRMS]
Marine/Coastal |
| Authors | | Top |
- Belmans, D.
- Van Laere, A.
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| Abstract |
Glycerol-3-phosphatase (EC 3.1.3.2.1) was studied by following the release of radioactive glycerol from L-(U-14C)glycerol-3-phosphate in Dunaliella tertiolecta enzyme extracts. The reaction showed a neutral pH optimum and had an absolute requirement for Mg2+. The substrate saturation curve was hyperbolic with an apparent Km value for glycerol-3-phosphate of 0.7 mM in the absence of phosphate. Inorganic orthophosphate was a competitive inhibitor of the enzyme with an estimated Kj of 0.1 mM. The glycerol-3-phosphatase reaction was blocked nearly completely by millimolar Ca2+ concentrations. Ca2+ inhibition did not depend on the presence of calmodulin in the reaction medium. The characteristics of glycerol-3-phosphatase are discussed in relation to the regulation of the cyclic glycerol metabolism in Dunaliella cells during periods of osmotic stress. |
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