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A small-angle x-ray scattering study of the quaternary structure of the 24-S component of the hemocyanins of Homarus vulgaris and Cancer pagarus
Bernard, H.; Pilz, I.; Meisenberger, O.; Witters, R.; Lontie, R. (1983). A small-angle x-ray scattering study of the quaternary structure of the 24-S component of the hemocyanins of Homarus vulgaris and Cancer pagarus. Biochim. Biophys. Acta 748(1): 28-33. dx.doi.org/10.1016/0167-4838(83)90023-7
In: Biochimica et Biophysica Acta. Elsevier: Amsterdam. ISSN 0006-3002, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Bernard, H.
  • Pilz, I.
  • Meisenberger, O.
  • Witters, R.
  • Lontie, R.

Abstract
    The quaternary structure of the 24-S components of the hemocyranins of Homarus vulgaris and Cancer pagurus was studied by small-angle X-ray scattering in solution at neutral pH. Their scatterng curves are very similar and could be fitted by the theoretical scatterng curves based on a model similar to the one proposed earlier for the hemocyanin of Astacus leptodactylus (Pils, I., Goral, K., Hoylaerts, M., Witters, R. and Lontie, R. (1980) Eur. J. Biochem. 105, 539–543). The following molecular parameters were obtained: H. vulgaris hemocyanin, radius of gyration = 7.25 nm, radius of gyration of the cross-section = 3.9 nm, maximum dimension = 23.5 nm, maximum dimension of the cross-section = 11.4 nm, volume = 1620 nm3; C. pagarus hemocyanin, radius of gyration = 7.10 nm, radius of gyration of the cross-section = 3.8 nm, maximum dimension = 23.0 nm, maximum dimension of the cross-section = 11.2 nm, volume = 1600 nm3.

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