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A cryptobiosis-specific 19S protein complex of Artemia salina gastrulae
De Herdt, E.; De Voeght, F.; Clauwaert, J.; Kondo, M.; Slegers, H. (1981). A cryptobiosis-specific 19S protein complex of Artemia salina gastrulae. Biochem. J. 194(1): 9-17. hdl.handle.net/10.1042/bj1940009
In: Biochemical journal. Biochemical Society: London. ISSN 0264-6021, more
Peer reviewed article  

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  • De Herdt, E.
  • De Voeght, F.
  • Clauwaert, J.
  • Kondo, M.
  • Slegers, H.

Abstract
    The postribosomal supernatant of Artemia salina cryptobiotic embryos contains a large quantity of a 19S protein complex. An amount of 3.6 mg/g of cysts is measured by immunoprecipitation with anti-(19S protein complex) antibody. The quantity of this complex decreases during further development to nauplius larvae to only 15% of the quantity present in cryptobiotic embryos. The complex was no longer detectable after 7 days of growth. The 27000-Mr protein subunit of the 19S complex is not synthesized by mRNA isolated from cryptobiotic embryos. The cryptobiosis-specific complex has Mr 573000 and 610000 as calculated from light-scattering and sedimentation-diffusion measurements respectively. The 19S homocomplex contains 20-23 27000-Mr proteins and has no function in the translation of homologous mRNA. From hydrodynamic data a hydration of 1.25 g of water/g of protein is calculated. The abundant presence of the 19S protein complex in cryptobiotic embryos and the absence of synthesis during development to nauplius larvae indicate a functional role during the cryptobiotic process in early embryogenesis. A role in maintaining the water content of the cytoplasm above a critical threshold during desiccation is suggested.

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