|Characterization of protein components of poly(A)-containing messenger ribonucleoproteins from cryptobiotic gastrulae of Artemia salina|Slegers, H.; De Herdt, E.; Kondo, M. (1979). Characterization of protein components of poly(A)-containing messenger ribonucleoproteins from cryptobiotic gastrulae of Artemia salina. Molecular biology reports 5(1-2): 65-69. dx.doi.org/10.1007/BF00777490
In: Molecular biology reports. ISSN 0301-4851, more
|Authors|| || Top |
- Slegers, H.
- De Herdt, E.
- Kondo, M.
Conditions for the isolation of intact poly(A)+mRNP from cryptobiotic gastrulae of A. salina are described. In the presence of Mg2+ ions nucleolytic cleavage occurs in vitro in the vicinity of the 3′-poly(A) segment of mRNP during the isolation procedure. The resulting two parts of poly(A)+mRNP complex are separated by thermal elution from oligo(dT)-cellulose affinity column. Analysis by SDS-gel electrophoresis of protein components associated with intact poly(A)+mRNP has revealed the existence of 20–30 S RNP complex containing five major proteins with Mr 68,000, 53,000, 50,000, 45,000 and 38,000, respectively, but completely lacking the poly(A)-specific Mr 76,000 protein.