|Effects of different calcium, magnesium, and zinc concentrations supplemented on hepatopancreatic cell proliferation of Kuruma prawn, Penaeus japonicus|Wang, H.; Cai, D.; Li, K.; Xu, H.; Yang, L.; Zhu, N. (2012). Effects of different calcium, magnesium, and zinc concentrations supplemented on hepatopancreatic cell proliferation of Kuruma prawn, Penaeus japonicus. Biol. Trace Elem. Res. 148(2): 198-202. dx.doi.org/10.1007/s12011-012-9366-0
In: Biological Trace Element Research: Official Journal of the International Association of Bioinorganic Scientists and The Official Journal of the World Biomedical Selenium Society. Humana Press Inc.: Clifton, N.J.,. ISSN 0163-4984, more
Penaeus japonicus; Hepatopancreatic; Cell culture; Ca2+; Mg2+; Zn2+
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The effects of different calcium (Ca2+), magnesium (Mg2+), and zinc (Zn2+) concentrations supplemented on hepatopancreatic cell proliferation of kuruma prawn, Penaeus japonicus was studied. The culture system consists of medium 199 (M 199) supplemented with 0.060 mol/L NaCl, 1.011 g/L glucose, 1,000 UI/ml penicillin, 1,000 μg/ml treptomycin, heat inactivated fetal calf serum (FCS) 20% for primary cell culture and 10% for subculture. The RNA/DNA ratio of the cell cultures was measured. The results show that the cell division of hepatopancreatic cells of P. japonicus was enhanced by the optimal concentration of inorganic salt (Ca2+, 1.0 g/L; Mg2+, 5.0 g/L; Zn2+, 80 μg/L). The hepatopancreatic cell culture system and improved culture conditions described here will be very useful for in vitro experiments to study viruses responsible for infections in shrimp leading to tremendous economic losses.