|Identification of heat shock proteins from bacteria by electrophoretic separation and nanoflow LC-MS/MS|Szanics, E.; Devreese, B.; Van Beeumen, J. (2004). Identification of heat shock proteins from bacteria by electrophoretic separation and nanoflow LC-MS/MS. Acta Alimentaria 33(4): 397-404. dx.doi.org/10.1556/AAlim.33.2004.4.10
In: Acta Alimentaria. Akadémiai Kiadó: Budapest. ISSN 0139-3006, more
heat shock protein; psychrophilic bacteria; two-dimensionalpolyacrylamide gel electrophoresis; automated nanoflow LC-MS/MS
|Authors|| || Top |
- Szanics, E.
- Devreese, B.
- Van Beeumen, J.
Examination of heat shock and PR ("pathogenesis-related") proteins is of special interest in food science. Many food allergens have a similar or the same structure as PR proteins, which are produced in the plants as a response to pathogenesis or certain environmental stresses. The protein set of the psychrophilic bacterium Shewanella hanedai was studied by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Gel patterns from control and heat-treated bacteria were evaluated by PDQUEST software. The differentially expressed proteins were excised from the gel and digested by trypsin. The tryptic peptides were analysed by nanoflow LC-MS/MS. On the basis of amino acid sequences obtained by this method, the proteins were identified by similarity searching in the protein database. Using this proteomic approach a heat shock and a 50S ribosomal protein were identified as the major heat induced proteins in Shewanella hanedai.