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Neuropeptidomic analysis of the brain and thoracic ganglion from the Jonah crab, Cancer borealis
Huybrechts, J.; Nusbaum, M.P.; Vanden Bosch, L.; Baggerman, G.; De Loof, A.; Schoofs, L. (2003). Neuropeptidomic analysis of the brain and thoracic ganglion from the Jonah crab, Cancer borealis. Biochem. Biophys. Res. Commun. 308(3): 535-544. dx.doi.org/10.1016/S0006-291X(03)01426-8
In: Biochemical and Biophysical Research Communications. ACADEMIC PRESS INC ELSEVIER SCIENCE: San Diego etc.. ISSN 0006-291X, more
Peer reviewed article  

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Keywords
    Cancer borealis Stimpson, 1859 [WoRMS]; Marine
Author keywords
    Q-TOF; MALDI-TOF imaging; mass spectrometry; peptidomics; Cancerborealis; orcokinin; FMRFamide-related peptides; allatostatins

Authors  Top 
  • Huybrechts, J.
  • Nusbaum, M.P.
  • Vanden Bosch, L.
  • Baggerman, G.
  • De Loof, A.
  • Schoofs, L., more

Abstract
    Mass spectrometric methods were applied to determine the peptidome of the brain and thoracic ganglion of the Jonah crab (Cancer borealis). Fractions obtained by high performance liquid chromatography were characterized using MALDI-TOF MS and ESI-Q-TOF MS/MS. In total, 28 peptides were identified within the molecular mass range 750–3000 Da. Comparison of the molecular masses obtained with MALDI-TOF MS with the calculated molecular masses of known crustacean peptides revealed the presence of at least nine allatostatins, three orcokinin precursor derived peptides, namely FDAFTTGFGHS, [Ala13]-orcokinin, and [Val13]-orcokinin, and two kinins, a tachykinin-related peptide and four FMRFamide-related peptides. Eight other peptides were de novo sequenced by collision induced dissociation on the Q-TOF system and yielded AYNRSFLRFamide, PELDHVFLRFamide or EPLDHVFLRFamide, APQRNFLRFamide, LNPFLRFamide, DVRTPALRLRFamide, and LRNLRFamide, which belong to the FMRFamide related peptide family, as well as NFDEIDRSGFA and NFDEIDRSSFGFV, which display high sequence similarity to peptide sequences within the orcokinin precursor of Orconectes limosus. Our paper is the first (neuro)peptidomic analysis of the crustacean nervous system.

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